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Structured Review

Promega phage vector lambda embl3
Phage Vector Lambda Embl3, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phage+vector+lambda+embl3/embl3/pmc02915743-194-30-34
Average 90 stars, based on 1 article reviews
phage vector lambda embl3 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Construct:

Article Title: Estrogen-dependent Transcription of the NEL-like 2 ( NELL2 ) Gene and Its Role in Protection from Cell Death
Article Snippet: .. A mouse genomic DNA library (Invitrogen) was constructed in Escherichia coli LE392 after ligating partial Sau3AI DNA digests of mouse genomic DNA (strain 129/Sv) at the BamHI site of a phage vector lambda EMBL3 (Promega, Madison, WI). .. For screening clones containing the m NELL2 gene, ∼1 × 10 7 phage plaques were transferred to Hybond N + membrane (Amersham Biosciences) and hybridized to a [α- 32 P]dCTP (50 μCi/μl, Amersham Biosciences)-labeled m NELL2 cDNA probe; the cDNA fragment (a 680-bp fragment spanning nucleotides 21–700 in m NELL2 mRNA) was cloned by PCR amplification based on the deposited DNA sequence (NCBI GenBank TM accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_016743","term_id":"133922560","term_text":"NM_016743"}} NM_016743 ).

Plasmid Preparation:

Article Title: Estrogen-dependent Transcription of the NEL-like 2 ( NELL2 ) Gene and Its Role in Protection from Cell Death
Article Snippet: .. A mouse genomic DNA library (Invitrogen) was constructed in Escherichia coli LE392 after ligating partial Sau3AI DNA digests of mouse genomic DNA (strain 129/Sv) at the BamHI site of a phage vector lambda EMBL3 (Promega, Madison, WI). .. For screening clones containing the m NELL2 gene, ∼1 × 10 7 phage plaques were transferred to Hybond N + membrane (Amersham Biosciences) and hybridized to a [α- 32 P]dCTP (50 μCi/μl, Amersham Biosciences)-labeled m NELL2 cDNA probe; the cDNA fragment (a 680-bp fragment spanning nucleotides 21–700 in m NELL2 mRNA) was cloned by PCR amplification based on the deposited DNA sequence (NCBI GenBank TM accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_016743","term_id":"133922560","term_text":"NM_016743"}} NM_016743 ).



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